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On the subsequent day, the wells were blocked (200 µL/well) with PBS/M (pH 7.4) for 1 h at room temperature and washed (250 µL/well) four times with PBS/T (pH 7.4)

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In clinical trials of Tirzepatide, a dual-acting GLP-1 and GIP agonist currently prescribed for Type 2 diabetes, obese patients lost 21 percent body mass in 1.4 years, surpassing the 15 percent reduction seen with Semaglutide