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The column was washed with 20 ml equilibration solution, and elution was performed with a linear salt gradient from 0 to 250 mmol/kg NaCl (20 mmol/kg Tris-hydroxymethyl amino-methane, 45 % (w/w) ethanol, pH 8.5) followed by 40 ml of isocratic elution with 250 mmol/kg NaCl

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