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Free PCSK9 in standards, controls and samples was captured by a biotinylated monoclonal anti-human PCSK9 antibody at 100 µg/mL (in-house) and detected by an Alexa-labelled PCSK9 Affinity Purified PAb antibody at 30 nM (R&D Systems) on a Gyrolab Bioaffy 1000 CD platform (Gyros AB)
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